F. Tranquart 1, B. Petit 1,2, E. Bihel 1, Y. Bohren 1, F. Yan 1, E. Allemann 2, M. Arditi 1, J. M. Hyvelin 1
1 BRACCO SUISSE SA – Plan Les Ouates, Switzerland, 
2 School of Pharmaceutical Sciences, University of Geneva, University of Lausanne – Geneva, Switzerland

Rapid restoration of vascular flow is the primary goal of acute stroke treatment, while improvement in patient’s outcome is the ultimate benefit of such treatments. Among different treatment schemes, sonothrombolysis has been used in in vitro tests, in preclinical trials and to a lesser extent in clinical trials, even though the exact underlying mechanism has not been fully clarified.

I. Todorov, T. Eftimov, I. Hadzhiangelov, V. Nakov, P. Simeonov, E. Stavrev, C. Ninov, K. Romanski, Al. Petkov
Department of Neurosurgery, Military Medical Academy – Sofia, Bulgaria

Objective: For more than two decades the neuronavigation was successfully used in the neurosurgery. Some shortcomings of the method have evoked the need of an appropriate intraoperative imaging to compensate for the registration and shifting bias and the intraoperative ultrasound is found to be a good option. To analyze the results of the usage of intraoperative ultrasound combined with neuronavigation in different fields of neurosurgery.

Material and Methods: Between the years 2008 and 2013, 240 surgeries under ultrasound/navigation guidance were performed in the Department of Neurosurgery of the MMA – Sofia. Patients were at an average age of 54±14.26 years, harboring oncologic (91.3%), vascular (6.7%), inflammatory (1.3%) and congenital (0.8%) diseases. Integrated ultrasound navigation system (Sonowand Invite) was used intraoperatively in different modes: ultrasound only – in 46cases; 3D ultrasound registration – in 72 cases; “classic” neuronavigation (based on preoperative images) – in 15cases; and ultrasound-based navigation – in the rest 107 (45%) cases.

S. Yoshikazu, R. Suzuki, Y. Oda, D. Omata, K. Maruyama
Laboratory of Drug and Gene Delivery Faculty of Pharma-sciences – Tokyo, Japan

Objective: The combination thrombolysis therapy with recombinant tissue plasminogen activator (rt-PA), microbubbles and ultrasound, was reported higher recanalization ratio in ischemic cerebrovascular disease. Ultrasound and microbubbles vibrate the fibrin net and accelerate thrombolysis. Recently, we developed novel liposomal submicron bubbles (Bubble Liposome (BLs)) containing ultrasound imaging gas, perfluoropropane. In this study, we developed BLs with Arg-Gly-Asp (RGD) sequence-containing peptides, which bind to the activated platelet glycoprotein IIb/IIIa complexes. The aim of this study was to examine the enhancing effects in ultrasonic thrombus imaging using these targeted BLs in vitro and in vivo.